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Introduction
In vitro cell culture of primary cells and cell lines is an invaluable tool in life science research, offering reproducibility, accessibility, and versatility across various applications including basic research and drug discovery. Cell lines have contributed to numerous scientific breakthroughs, including vaccine development and informing our fundamental understanding of cancer and other disease states. Key advantages of cell lines include their cost-effectiveness compared to primary cells or animal models as well as providing a virtually unlimited supply of experimental material in a consistent and reproducible manner. Cell lines are relatively easy to generate and use for large-scale or high-throughput screening studies. They are widely used in pharmaceutical and drug discovery research serving as in vitro tools for screening of potential drug candidates, studying drug response and mechanism of action, and even predicting clinical responses to targeted therapies. Recent advancements in genome editing technology, synthetic biology platforms and 3D cell culture techniques have further enhanced their utility.
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