Ultra-High Coverage of the Serum Proteome Using a Multi-Nanoparticle Based Workflow
American Society of Mass Spectrometry – Florian Flenkenthaler, et al.
This poster demonstrates ultra-high coverage of serum proteins with over 5,000 protein groups identified in a 105-sample cohort and over 3,000 protein groups identified on average per sample. Proteins were quantified at 3-5 fold higher levels compared to depleted and un-depleted serum, with 30% enhanced coverage of low abundant cytokines. This data supports a comprehensive serum proteomics workflow that can be used for patient stratification as well as potential identification of novel biomarkers which are not accessible through other strategies.
Introduction
For years, the ultra-wide abundance range of proteins in plasma and serum has limited clinical applications by unbiased mass spectrometry based proteomics to just a few hundred high-abundance proteins. We show that by using the proprietary multi-nanoparticle based Proteograph technology to enrich low abundant proteins from serum, these challenges can now be efficiently addressed allowing detection of more than 5,000 proteins in a patient cohort with more than 3,000 proteins in individual patient serum samples.
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