December 14, 2022 | Customer Poster
Integrated Plasma Multi-Omics Using Nanoparticle Technology and Single-Shot Capillary MS
Human Proteome Organization – Corinne E. Moss, et al.
This poster demonstrates how the Proteograph workflow enables, deep, and rapid multi-omic analysis of plasma in a study that resulted in ~5X better protein groups coverage versus neat plasma digestion workflow with a single injection DDA LC-MS workflow with FAIMS Pro interface on the Orbitrap MS system.
Introduction
Plasma Proteomics with Seer
- Plasma is one of the most biomolecule-rich sample types
- Plasma proteins exist at concentrations that vary by 10 orders of magnitude, creating a huge dynamic range problem
- Seer uses Nanoparticle (NP) technology to differentially select proteins from human plasma
- Proteins differentially aggregate to each of the 5 NP’s based on the physiochemical properties of the beads
- Plasma samples can be processed in an automated workflow using the Seer Proteograph Suite
Multi-omics using MOST
- Simultaneous analysis of proteins, lipids, and metabolites would provide and exceptionally powerful and efficient means of biomolecule analysis
- Mass Spectrometry is well suited to analyze many classes of molecules
- Potential applications include biomarker analysis, precision medicine, diagnostics, metabolomic and proteomic studies
- Multi-Omic Single Shot Technology (MOST) enables proteomic and lipidomic analysis from a singular MS run using a modified LC gradient
- Recently, MOST has been adapted for nano-flow LC-MS
- MOST technology is compatible with proteomic samples from many cell types and species
Combining -omic Strategies
- Expansion and optimization of plasma biomolecule analysis necessitates a depletion of high abundance proteins
- Plasma samples processed in the Seer Proteograph Suite result in peptide samples for a broad selection of plasma proteins
- NP peptide samples can be analyzed in tandem with lipid samples using MOST technology
- Peptide separations can be enhanced using a high-field asymmetric waveform ion mobility spectrometry (FAIMS) device which separates ions before transfer into the Mass Spec based on their mobility in various electric fields
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