Nanoparticle-Enabled Plasma Proteomics of a Mouse Atherosclerosis Model

In this preclinical study, researchers applied the Proteograph^® Product Suite to deeply profile plasma from a murine model of atherosclerosis—overcoming the limitations of conventional workflows that often fail to capture low-abundance proteins in small-volume samples. The approach enabled consistent quantification of over 5,000 proteins per sample from just 75 µL of plasma, representing a >10-fold increase in proteome depth compared to conventional methods.

The resulting dataset revealed proteomic shifts that varied over time and with dietary intervention, including elevation of the MMP12 biomarker that is potentially linked to plaque instability. In addition, 120 coronary artery disease–associated human proteins were detected in the mouse plasma, highlighting the translational power of this approach. These findings demonstrate how nanoparticle-enabled proteomics can provide mechanistic insight and cross-species relevance—bridging preclinical discovery with human cardiovascular research.