Unbiased and Deep Proteomic Analysis of Secretome Samples Using the Proteograph XT Workflow
This application note illustrates the capability of the Proteograph XT workflow for comprehensive proteomic characterization of conditioned media secretomes, highlighting advantages achieved without the need for traditional preprocessing and sample manipulation protocols.
Synopsis
Easily accessible biofluids like plasma, serum, and conditioned media (CM) serve as important sample types for proteomic analysis in biomarker discovery, biotherapeutic properties and understanding human health and disease states. We evaluated the application of the Proteograph™ XT workflow, a standardized, automated, nanoparticle-based deep proteomics workflow optimized for plasma samples, to interrogate proteomic performance in a variety of secretome sample types under various cell line conditions, with no additional modifications to the standard protocol. We demonstrate the feasibility of using the standard Proteograph XT workflow to support comprehensive proteomic characterization of conditioned media secretomes.
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