The Utility of Nanoparticle Protein Coronas for Studying the Plasma Glycoproteome
This collaboration between Seer and Protein Metrics presented at the American Society for Mass Spectrometry describes the formation of NP protein coronas that enable analysis of subpopulations of the glycoproteome without the need for subsequent, glycopeptide-specific enrichment.
Introduction
A novel strategy using nanoparticle protein coronas, which enables the detection of low abundance proteins and improved plasma proteome coverage, was recently reported. Nanoparticles enable robust and reproducible measurement of the plasma proteome, which assists in the discovery of novel protein biomarkers. Since nanoparticle coronas differentially interrogate complex samples at the proteoform level (i.e. native proteins) and plasma proteins are often glycosylated, we investigated whether this strategy could also provide a robust route towards the plasma glycoproteome without the need for subsequent enrichment of glycosylated peptides. Protein glycosylation states can provide diagnostic evidence and a complementary insight when the total protein abundance is uninformative. Improved methods for profiling the plasma glycoproteome thus harbor the potential to accelerate biomarker discovery.
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